| セッション情報 |
International Forum 1(Gastrointestinal tract) 2.Pathogenesis of NSAIDs-gastropathy
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| タイトル |
IF1-2-II (Plenary presentation) Role of microRNAs on anti-cancer effect of a selective COX-2 inhibitor in human gastric cancer cells
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| 演者 |
Yoshimasa Saito（Department of Internal MedicineSchool of MedicineKeio UniversityTokyoJapan） |
| 共同演者 |
Hidekazu Suzuki（Department of Internal MedicineSchool of MedicineKeio UniversityTokyoJapan）, Toshifumi Hibi（Department of Internal MedicineSchool of MedicineKeio UniversityTokyoJapan） |
| 抄録 |
Background and AimmicroRNAs （miRNAs） are small non－coding RNAs that function as endogenous post－transcriptional si－lencers of target genes． miRNAs play important roles during the development of cancersuggesting thatmiRNAs can be potential therapeutic targets． On the other handinhibition of cyclooxygenase－2 （COX－2）has been proposed to be a potential mechanism for the chemoprevention of gastrointestinal tumors bynonsteroidal anti－inflammatory drugs． The aims of this study are to analyze the expression profiling ofmiRNAs after treatment of human gastric cancer cells with COX－2 inhibitor and to clarify the roles ofmiRNAs in gastric cancer cells treated with COX－2 inhibitor．Materials and MethodsAGS human gastric cancer cells were exposed to 50pM of a selective COX－2 inhibitorcelecoxib． Aftertreatmenttotal RNAs were extracted and miRNA expression profiling was analyzed by miRNA microar－ray analysis． Expression of miRNAs that were differentially expressed was confirmed by real－time PCR．Expression of target of miRNAs was analyzed by Western blot analysis．ResultsExpression profiling of AGS cells revealed that 34 out of 720 miRNAs were differentially expressed bytreatment with celecoxib． ln particularmiR－29 and miR－181 were upregulated more than 4－fold in AGScells treated with celecoxib． Recentlyoncogene TCLエis shown to be a target of miR－29 and miR－181． Ex－pression of TCLI was downregulated after treatment with celecoxib．ConclusionThese rcsults suggest that miRNAs play critical roles on anti－cancer effect of a selective COX－2 inhibitorin human gastric cancer cells |
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