| セッション情報 |
Poster Presentation(GI)
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| タイトル |
IF-P1-8 Assessment of Bacterial Diversity Associated with Colorectal Neoplasia
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| 演者 |
Alison Teo（Division of Applied MedicineInstitute of Medical SciencesUniversity of AberdeenUK） |
| 共同演者 |
Georgina L. Hold（Division of Applied MedicineInstitute of Medical SciencesUniversity of AberdeenUK）, Mairi E. Hope（Division of Applied MedicineInstitute of Medical SciencesUniversity of AberdeenUK）, Susan H. Berry（Division of Applied MedicineInstitute of Medical SciencesUniversity of AberdeenUK）, Emad M. El-Omar（Division of Applied MedicineInstitute of Medical SciencesUniversity of AberdeenUK） |
| 抄録 |
Introduction ： The human gut is a complex microbial ecosystem unique to every individual Dysbiosis （dis－turbance of this ecosystem） is thought to contribute to chronic inflammation in several disease models． Werecently showed that chronic inflammation plays a role in the pathogenesis of sporadic colorectal neopla－sia． We hypothesised that the colonic microbiota is an important一 driver of this inflammation－induced neo－plastic progression．Aim ：’ To compare bacterial diversity in adenomas and paired adjacent normal colonic・ tissue．Methods ： Paired polyp／adj acent mucosa biopsies were obtained from 72 patients who had at least onelarge adenoma （＞lcm）． Heaithy controls had six mucosal sites biopsied． Denaturing gradient gel electro－phoresis was used to assess bacterial diversity in adenomas／adj acent mucosa pairs an d across the colon incontrols． Twenty tissue pairs were selected for in depth bacterial diversity assessment by 454 Titaniumnext generation sequencing．Results ： Healthy controls showed little variation in mucosal bacterial populations across the colon． ln con－trast460／o of patients showed differences in microbial diversity by DGGE analysis between the adenoma／’adj acent normal tissue （210／o had 1imited while 790／o had extensive differences in microbial diversity）． Se－quence analysis showed that the most commonly identi’fied bacterial phyla in the healthy colon were Bac－teroidetesFirmicutes and Proteobacteria． ln contrastFusobacteria species were associated with neo－plastic lesions．Conclusion ： Our novel findings suggest that significant dysbiosis occurs in colorectal polyps an d th1’s maydrive some towards cancer by inducing infiammation． The identification of ・a “harmfu1’” pro－infiammatorymicrobiota offers the potential for therapeutic interventions with probio七icprebiotic or antibiotic means． |
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