| セッション情報 |
シンポジウム9
疾患モデル動物を用いた消化器病研究の最前線
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| タイトル |
S9-2 Cytoglobin-deficient mice―a novel animal model to study liver and lung carcinogeneis
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| 演者 |
Thi Thanh Thuy Le（大阪市立大学大学院医学研究科肝胆膵病態内科学） |
| 共同演者 |
河田則文（大阪市立大学大学院医学研究科肝胆膵病態内科学） |
| 抄録 |
Aim ： Cytoglobin （Cygb） is a recently discovered vertebrate gtobin withunknow－n function． To study the biological function of Cygb in vivowegenerated Cygb knockout mice and investigated their susceptibility to NN－diethylnitrosamine （DEN） 一induced turnorigenesis． Methods ： Micelackng exon 1 of the Cygb gene were generated using the lox－P system．Four－week－old’Cygb” ’ （wi’ld－type）Cygb ”’ （heterozygote）and Cygb’ ’（homozygote） male mice were administrated DEN in drinking water atthe dose of 25 pp1n for 25 weeks or O．05 ppm for 36 weeksTumor forma－tion and the role Qf Cygb in DEN－mediated carcinogenesis were ana－Iyzed． Results ： Cygb deficiency prometed the DEN－induced deveEop－rnent of liver and lung tumors． All Cygb．． and Cygb”． mice treated wi出as ppm DEN exhibited liver tumorscompared with 44．4％ of their wild－type coun’terparts． Lung tumors were present only in Cygb－deficientmice． More than 40％ of Cygb ” mice developed liver and iung tumorgatthe non－toxic dose of DEN（OP5 ppm）wbich did not induce tu皿ors inwild－type miceCygb loss was associated with increased cancer ce皿pr（ンliferation． elevated Erk and Akt activationover－expression of tumor pro－moting cytokineshepatic collagen accumulationand increased oxidativestress． Conelusions ： This study is the first to report that Cygb deficiencyinduced susceptibility te cancer development in the liver as well as thelungs of mice under DEN treatment Cygb－deficient mice can be a usefulanimal rnodel to study liver and lungs tumorigenesis． Globins such asCygb wM thus shed new light on the biology of organ carcinogenesis |
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