| セッション情報 | The 2nd JSGE International Topic Conference3）Gastric Cancer；From the View of Helicobacter pylori-related Inflammation |
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| タイトル | IT3-1：Helicobacter pylori：Mechanisms for Adhesion to the Gastric Mucosa |
| 演者 | Arnqvist Anna（Department of Medical Biochemistry and Biophysics, Umea University, Sweden） |
| 共同演者 | |
| 抄録 | Helicobacter pylori infects the human stomach of half the world population and represents one of the most frequent causes of inflammatory processes in the stomach. The association of H. pylori infection and development of peptic ulcer disease and gastric cancer is well established. Adhesion to the gastric epithelium is an important factor for maintenance of the infection. In healthy gastric epithelium, H. pylori bind to ABO/Lewis b blood group antigens via its BabA adhesin. As a result of the infection and the subsequent inflammation, expression of the sialyl-Lewis x/a antigens increases. These antigens are also receptors for the H. pylori SabA adhesin. The continuous local changes of the glycosylation pattern require adaptation of the H. pylori adhesion properties to maintain the infection. Adhesion can also be devastating exposing H. pylori to host immune responses. This requires a balanced relation between expression of the adherence properties and the host immune responses. We used 5’RACE, promoter reporter fusions and in vitro mutagenesis together with gel mobility shift assays and DNase foot printing to functionally characterize the molecular details that operate at the level of transcription to fine tune expression of the inflammation associated SabA adhesin. Our study also includes details on how sabA transcription is regulated in response to pH. We also report on the characterization of H. pylori outer membrane vesicles（HpOMV）and in particular virulence associated factors and mechanisms for uptake of HpOMV by the host cell. Our results provide molecular details for the basic transcriptional mechanisms that operate to balance sabA gene expression and a description of OMVs as possible alternative pathway for delivery of host effector molecules. |
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