| セッション情報 |
The 2nd JSGE International Topic Conference Poster Session
Upper GI：Basic Approach
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| タイトル |
IT-P-07：Next-generation Sequencing can be a Useful Tool to Determine Mutations Related to Drug Resistance
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| 演者 |
Tran Binh（Department of Environmental and Preventive Medicine, Oita University, Japan） |
| 共同演者 |
Shiota Seiji（Department of Environmental and Preventive Medicine, Oita University, Japan）, Suzuki Rumiko（Department of Environmental and Preventive Medicine, Oita University, Japan）, Kwon Dong（Department of Environmental and Preventive Medicine, Oita University, Japan）, Yamaoka Yoshio（Department of Environmental and Preventive Medicine, Oita University, Japan） |
| 抄録 |
Background：Antibiotic resistance, especially for clarithromycin is the most important factor causing failure of treatment of H. pylori infection. Although the clarithromycin resistance is associated with point mutations in the 23S rRNA, it is not clear whether there are other mutations associated with the resistance or not. In this study, we first analyzed the genome profile for clarithromycin resistance using next-generation sequencer. Methods：Two types of different clarithromycin resistant strains were separately obtained from strain 26695 under the exposure of low concentration of clarithromycin in vitro. The genome sequences of three types of H. pylori（wild-type and two clarithromycin resistant strains）were determined by a next-generation sequencer（Illumina HiSeq2000）. Candidate mutations were confirmed by polymerase chain reaction（PCR）and DNA sequencing. Results：Clarithromycin resistant strains showed 20 mutations（SNPs or deletion）compared with wild-type strain. Among 20 mutations, 8 mutations in 8 genes were confirmed by PCR and DNA sequencing. Clarithromycin resistant strains contained the well-known mutations the 23S rRNA（A2143G）. On the other hand, there were 6 mutations in 6 genes out of 23S rRNA, suggesting the presence of novel candidate genes for clarithromycin resistance. Conclusions：Not only point mutations in the 23S rRNA, but also novel mutations in 6 genes might be associated with clarithromycin resistance. |
| 索引用語 |
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