セッション情報 The 2nd JSGE International Topic Conference Poster Session

Colitis-Associated Cancer

タイトル IT-P-12:

P53 IHC Resolves Difficulty of Pathological Diagnosis in Indefinite Lesions for Neoplasia in UC;Using Image Processing Software Analysis

演者 Okamoto Yosuke(Department of Surgical and Molecular Pathology, Dokkyo Medical University of Medicine, JapanDELIMITERDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University Omori Medical Center, Japan)
共同演者 Kobayashi Shunsuke(Department of Surgical and Molecular Pathology, Dokkyo Medical University of Medicine, JapanDELIMITERDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University Omori Medical Center, Japan), Fujimori Takahiro(Department of Surgical and Molecular Pathology, Dokkyo Medical University of Medicine, Japan), Itabashi Michio(Department of Surgery 2, Tokyo Women’s Medical University, Japan), Kameoka Shingo(Department of Surgery 2, Tokyo Women’s Medical University, Japan), Iizuka Bunei(Department of Gastroenterology, Tokyo Women’s Medical Center, Japan), Shigaki Koutarou(Department of Organ Regulatory Surgery, Fukushima Medical University, Japan), Akimoto Naohiko(Department of Surgical and Molecular Pathology, Dokkyo Medical University of Medicine, Japan), Yamaguchi Takeshi(Department of Surgical and Molecular Pathology, Dokkyo Medical University of Medicine, JapanDELIMITERDepartment of Surgery 1, Dokkyo Medical University, Japan), Ichikawa Kazuhito(Department of Surgical and Molecular Pathology, Dokkyo Medical University of Medicine, Japan), Tomita Shigeki(Department of Surgical and Molecular Pathology, Dokkyo Medical University of Medicine, Japan), Igarashi Yoshinori(Division of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University Omori Medical Center, Japan)
抄録 The incidence of colorectal neoplasia is increased in patients with long-standing and extensive ulcerative colitis(UC). To improve the prognosis of patients with UC-associated neoplasia, it is important to diagnose it at pre-neoplastic state. However, not only UC-associated pre-neoplasia but also neoplasia is often difficult to detect endoscopically and to discriminate from regenerative epithelium pathologically. In this study, we investigated abnormality of p53 gene using immunohistochemistry(IHC)in colectomy specimens from seven patients with UC-associated neoplasia between 2010 and 2011 for discrimination of pre-neoplasia and regenerative epithelium. Each section was stained with HE and mucosal lesions were graded morphologically according to ‘Histological classification of the neoplastic epithelium arising in UC’ proposed by the Research Committee of Inflammatory Bowel Disease at the Ministry of Health and Welfare of Japan. These sections were examined using P53 IHC and image processing software analysis. The result was compared with morphological evaluation. P53 positive cells can be detected not only in neoplasia but also in indefinite lesion for neoplasia(IFN). Result;P53 was expressed as the percentage of positive cells relative to the total number of cells in the basal half of the glands counted under high power magnification. The P53 expression in UC-III(0.78, range 0.69-0.84)was increased compared to that observed in UC-IIa(0.24, range 0.20-0.31). Conclusion;P53 high-positive glands in IFN may be a candidate for UC-associated neoplasia.
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