||The 2nd JSGE International Topic Conference Poster Session
Macrophage Colony-stimulating Factor Plays a Pivotal Role in Hepatocarcinogenesis in Human and Mice
||Kono Hiroshi（First Department of Surgery, University of Yamanashi, Japan）
||Hara Michio（First Department of Surgery, University of Yamanashi, Japan）, Furuya Shinji（First Department of Surgery, University of Yamanashi, Japan）, Hirayama Kazuyoshi（First Department of Surgery, University of Yamanashi, Japan）, Fujii Hideki（First Department of Surgery, University of Yamanashi, Japan）
||Aim；The purpose of this study was to investigate the role of macrophage colony-stimulating factor（M-CSF）in hepatocarcinogenesis. Materials and Method；In animal studies, op/op and their littermate mice（LM）were intraperitoneally injected with diethylnitrosamine（DEN）to induce hepatocellular carcinoma, and tumor incidence rate was assessed. Distribution of Mφs and angiogenesis were evaluated. Isolated vascular endothelial cells（VEC）were co-cultured with or without Kupffer cells in presense with M-CSF and their cell proliferations were assessed. In human studies, the expression of M-CSF, population of macrophages（Mf）, and angiogenesis were assessed in tumor and peritumoral liver tissue from 55 patients who had undergone hepatectomy for histologically proven HCC. Prognostic values of these and other clinicopathologic factors were evaluated. Result；Tumor incidence was significantly reduced in the op/op compared with the LM. Angiogenesis increased in peritumor and intratumor in the LM compared with the op/op mice. Under isolated VEC co-cultured with Kupffer cells in present or absent with M-CSF, the proliferation of VEC was greatest in VECs cultured with the KC and M-CSF. In human studies, high expression of peritumoral M-CSF, Mf density, and angiogenesis were independent prognostic factors for disease free survival. Conclusions；M-CSF is involved in hepatocarcinogenesis in animal model and in human.