セッション情報 The 2nd JSGE International Topic Conference Poster Session

Virus, CRP and Anaphylatoxin

タイトル IT-P-27:

An Association between Quasispecies Nature of Hepatitis C Virus Core Region and Disease Progression Analyzed by Deep Sequencing

演者 Maekawa Shinya(First Department of Internal Medicine, University of Yamanashi, Japan)
共同演者 Miura Mika(First Department of Internal Medicine, University of Yamanashi, Japan), Komatsu Nobutoshi(First Department of Internal Medicine, University of Yamanashi, Japan), Tatsumi Akihisa(First Department of Internal Medicine, University of Yamanashi, Japan), Asakawa Yukiko(First Department of Internal Medicine, University of Yamanashi, Japan), Takano Shinichi(First Department of Internal Medicine, University of Yamanashi, Japan), Sato Mitsuaki(First Department of Internal Medicine, University of Yamanashi, Japan), Shindo Kuniaki(First Department of Internal Medicine, University of Yamanashi, Japan), Amemiya Fumitake(First Department of Internal Medicine, University of Yamanashi, Japan), Nakayama Yasuhiro(First Department of Internal Medicine, University of Yamanashi, Japan), Inoue Taisuke(First Department of Internal Medicine, University of Yamanashi, Japan), Sakamoto Minoru(First Department of Internal Medicine, University of Yamanashi, Japan), Enomoto Nobuyuki(First Department of Internal Medicine, University of Yamanashi, Japan)
抄録 Background & Aims:Core amino acid(a.a.)70 of hepatitis C virus(HCV)known for the association with interferon response has been recently suggested for its correlation with the liver disease progress. In the present study, quasispecies state of HCV core gene was investigated by ultra-deep sequencing. Methods:The subjects comprised 85 patients infected with genotype 1b-HCV(CH, 28;liver cirrhosis(LC), 31;hepatocellular carcinoma(HCC), 26). From sera of these patients, deep sequencing of the HCV core gene was carried out with Roche 454 GS Junior pyrosequencer. Results:With a plasmid containing an HCV clone sequence(pCV-J4L6S), the background error rate accompanied by the pyrosequencing procedure including PCR was calculated as 0.027%/base. Deep sequencing of the core region with clinical samples showed existence of a mixture of“mutant-type”Q/H and“wild-type”R at core a.a. 70 position in most cases(96.3%), and the mixture ratio increased as liver disease advanced to LC and HCC. Meanwhile, phylogenetic analysis on the almost whole core region revealed that HCV isolates form distinct subgroups according to the status of mutation at core a.a 70 in each patient. Conclusions:Core a.a 70 mixture ratio analyzed by deep sequencing reflected the status of liver diseases, and moreover, molecular evolution of whole core gene was influenced by the core a.a. 70, suggesting the core a.a.70 is a significant determinant regulating disease progression in genotype-1b CH-C.
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