| 共同演者 |
Makino Naohiko（Department of Gastroenterology, Yamagata University School of Medicine, Japan）, Tozawa Tomohiro（Department of Gastroenterology, Yamagata University School of Medicine, Japan）, Matsuda Akiko（Department of Gastroenterology, Yamagata University School of Medicine, Japan）, Ikeda Yushi（Department of Gastroenterology, Yamagata University School of Medicine, Japan）, Kakizaki Yasuharu（Department of Gastroenterology, Yamagata University School of Medicine, Japan）, Saito Yoshihiko（Department of Gastroenterology, Yamagata University School of Medicine, Japan）, Kawata Sumio（Hyogo Prefectural Nishinomiya Hospital, Japan）, Ueno Yoshiyuki（Department of Gastroenterology, Yamagata University School of Medicine, Japan） |
| 抄録 |
Introduction：Pancreatic cancer is increasing yearly in Japan, and diabetes has the highest incidence among diseases that are complications of pancreatic cancer. Meanwhile, it has become evident that increased expression of osteopontin（OPN）is observed in patients suffering from diabetes and obesity. The purpose of this study was to investigate the effect of high glucose and high insulin culturing conditions on human pancreatic duct epithelial cell line（HPDE-6）and investigate the OPN expression under the same conditions. Materials and Method：Glucose culturing conditions（6mM）, high glucose culturing conditions（30mM, 60mM）, and high insulin culturing conditions（0.1nM, 1nM）were combined and cultured using HPDE-6. Outcome：Cell proliferation was significantly accelerated under high glucose culturing conditions compared to cell proliferation of HPDE-6 under 6mM glucose culturing conditions, and was more prominent under high insulin culturing conditions. In the same manner, OPN mRNA expression also significantly increased. In comparison with 6mM glucose culturing conditions, the 8-OHdG in DNA and SOD2 mRNA expression level and LDH activity in culture supernatant increased under high glucose culturing conditions. Conclusion：Accelerated HPDE-6 cell proliferation and increased OPN expressions are observed under high glucose and high insulin culturing conditions. Furthermore, increased oxidative stress and cytotoxicity of HPDE-6 are observed under high glucose culturing conditions. |
