| セッション情報 |
International Session2(肝臓学会・消化器病学会合同)
Gene therapy and cell therapy through the liver; current aspects and future prospects
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| タイトル |
肝IS2-1指：The development of a less-invasive liver regeneration therapy using cultured bone marrow derived cells for cirrhotic patients
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| 演者 |
T. Takami（Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine） |
| 共同演者 |
S. Terai（Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine）, I. Sakaida（Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine） |
| 抄録 |
We have developed autologous bone marrow cell infusion (ABMi) therapy, which was a method of liver regeneration for cirrhotic patients using non-cultured autologous whole bone marrow (BM) cells, and reported the efficacy and safety of this approach. However, ABMi therapy involves BM aspiration under general anesthesia, and is not indicated for patients for whom general anesthesia is difficult. We therefore aimed to develop a less-invasive liver regeneration therapy in which cells having curative effects on liver cirrhosis are isolated and cultured from a small amount of autologous BM aspirated under local anesthesia and infused back into the same patient, showing that the indications would be able to be expanded. We revealed that the infusion of cultured human BM-derived CD73/CD90/CD105 positive and CD45 negative cells with fetal bovine serum-containing D-MEM medium, which were having mesenchymal characteristics, reduced hepatic fibrosis in the immunodeficient cirrhotic mice, consistent with the reduced oxidative stress in human stellate cells co-cultured with human mesenchymal stem cells in vitro. For the clinical trial using cultured BM-derived mesenchymal stem cells, we are preparing safety evaluation guidelines and a system conforming to Standard Operating Procedure at a Good Manufacturing Practice-grade cell-processing center in our university hospital. Here, we present the current status and prospects for our liver regeneration therapy using autologous BM-derived cells. |
| 索引用語 |
Liver cirrhosis, Bone marrow cells |
