| 抄録 |
Random integration of conventional gene delivery vectors such as viruses, plasmids, P1 phage-derived artificial chromosomes, bacterial artificial chromosomes and yeast artificial chromosomes can be associated with transgene-silencing. Furthermore, integrated sequences can activate oncogenes adjacent to the insertion site resulting in the activation or silencing of transgenes. Human artificial chromosome(HAC) is exogenous mini-chromosome artificially created by chromosome engineering in the DT40 cells. Various HACs exhibit several potential characteristics desired for an ideal gene delivery vector, including stable episomal maintenance and the capacity to carry large genomic loci with their regulatory elements, thus allowing the physiological regulation of the introduced gene in a manner similar to that of native chromosomes. In addition, multiple acceptor sites were introduced into the HAC. In this meeting, I introduce methods for constructing HAC vectors and potential characteristics of HACs, and various applications for basic and translational researches, e.g., gene function, iPS production,and gene- and cell-therapies. |
